After a marker is linked to within 1 cM of a disease gene, chromosome walking can be used to clone the disease gene itself. A probe is first constructed from a genomic fragment identified from a library as being the closest linked marker to the gene. A restriction fragment isolated from the end of the clone near the disease locus is used to reprobe the genomic library for an overlapping clone. This process is repeated several times to walk across the chromosome and reach the flanking marker on the other side of the disease- gene locus. (Source: see Fig. 11.)

Return to text

The Medical and Public Health Law Site
The Best on the WWW Since 1995!
Copyright as to non-public domain materials
See DR-KATE.COM for hurricane and disaster preparation
See WWW.EPR-ART.COM for photography of Southern Louisiana and Hurricane Katrina
Edward P. Richards, III, JD, MPH
Webmaster